MedicalResearch.com Interview with:
Héctor Peinado PhD
Microenvironment and Metastasis Laboratory
Molecular Oncology Program
Spanish National Cancer Research Center
MedicalResearch.com: What is the background for this study? What are the main findings?
Response: In this study we detected for the first time BRAF mutation by liquid biopsy in melanoma stage III patients that underwent lymphadenectomy. We obtained a novel biofluid from the drainage implanted 24-48 hours post-lymphadenectomy, called exudative seroma, and profiled BRAF mutation in circulating free DNA and extracellular vesicles.
Those patients positive for BRAF mutation in the seroma had increased risk of relapse, therefore we believe that this technique identifies patients at risk of relapse by identifying residual disease.
MedicalResearch.com: What should readers take away from your report?
Response: I would explore any material obtained post-surgery as means of residual disease so we can develop further test to improve prognosis.
MedicalResearch.com: What recommendations do you have for future research as a result of this work?
Response: This test was performed in melanoma patients, but could be applicable to any tumor type where lymphadenectomy of sentinel lymph node drainage is implanted. We are also developing test for plasma.
Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAFV600E mutation
Susana García-Silva, Alberto Benito-Martín, Sara Sánchez-Redondo, Alberto Hernández-Barranco, Pilar Ximénez-Embún, Laura Nogués, Marina S. Mazariegos, Kay Brinkmann, AnaAmor López, Lisa Meyer, Carlos Rodríguez, Carmen García-Martín, Jasminka Boskovic, RocíoLetón, Cristina Montero, Mercedes Robledo, Anna Szumera-Ciećkiewicz, Iwona Kalinowska, Johan Skog, Mikkel Noerholm, Javier Muñoz, Pablo L. Ortiz-Romero, Yolanda Ruano, José L.Rodríguez-Peralto, Piotr Rutkowski, Héctor Peinado
Journal of Experimental Medicine Apr 2019, jem.20181522; DOI: 10.1084/jem.20181522
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