Author Interviews, COVID -19 Coronavirus / 09.09.2020

MedicalResearch.com Interview with: Claudia Alteri, PhD Assistant Professor (RTD-B) in Microbiology And Clinical Microbiology Department of Oncology and Hemato-Oncology University of MilanoMedicalResearch.com: What is the background for this study? Response: In the context of SARS-CoV-2 diagnosis, laboratories play a critical role in confirming the initial clinical suspicion of this disease, as confirmation of SARS-CoV-2 presence is essential to ensure the prompt initiation of containment and treatment protocols. This is of utmost importance to avoid further spread of the pandemic, and to assure the best clinical and therapeutic management of the infected patients in the hospital setting. Unfortunately, currently used rtPCR assays lack of the necessary sensitivity to identify all cases of SARS-CoV-2 infection (20% of false negative results [Li D et all, Korean J Rad 2020; Zhao et all CID 2020]). Complementary laboratory assays are therefore strongly needed. Droplet Digital PCR (ddPCR) is a highly sensitive assay for the direct detection and quantification of DNA and RNA targets. It has been increasingly used in infectious disease settings, especially thanks to its ability to consistently and reliably detect down to few copies of viral genomes. Standing the necessity of a limitation (as much as possible) of false negative results in COVID-19 diagnosis, the use of ddPCR could provide a critical support. In the context of COVID-19 diagnosis, two recent studies highlight the good performances of ddPCR in detecting low viral load samples (Suo T MedRxiv 2020; You F MedRxiv 2020)(more…)
Author Interviews, BMJ, COVID -19 Coronavirus / 03.09.2020

MedicalResearch.com Interview with:corona virus-Covid19Dr. Francesco Venturelli Servizio di Epidemiologia Direzione Sanitaria - Azienda USL-IRCCS di Reggio Emilia Padiglione Ziccardi, Via Amendola MedicalResearch.com: What is the background for this study? Response: Contact tracing and isolation for people testing positive for SARS-CoV-2 are two of the main strategies to limit the viral spread and contain the current pandemic. Long persistence of viral RNA detected by RT-PCR on nasopharyngeal swabs is commonly reported, while its correlation to virus viability is still debated.MedicalResearch.com: What are the main findings?Response: The study showed that in people with COVID-19, the median time between symptoms onset and viral clearance at RT-PCR was 36 days. Moreover, an overall 20% risk of “false negative” results at RT-PCR was observed, decreasing with time from diagnosis. (more…)