MedicalResearch.com Interview with: Dr. Akihito Ishigami Ph.D. Vice President of the Division of Biology and Medical Sciences Tokyo Metropolitan Institute for Geriatrics and Gerontology, Japan MedicalResearch.com: What is the background for this study? Response: Keratinocyte differentiation is a highly regulated process essential for the stratified structure of the epidermis and must be balanced with cell proliferation. While Vitamin C (VC) is a known cofactor for DNA demethylation enzymes, its specific role in DNA demethylation during keratinocyte differentiation was unclear. This study aimed to evaluate VC's role in the epigenetic regulation of epidermal proliferation and differentiation using a human epidermal equivalent model to understand how VC affects gene expression via DNA demethylation changes.