25 Aug American CryoStem – CRYO – Allows Individuals to Collect, Freeze and Store Their Stem Cells

MedicalResearch.com Interview with:

John Arnone

John Arnone
Chairman and CEO
American CryoStem Corporation

MedicalResearch.com: What is the background for this your company American CryoStem?

Response: American CryoStem Corporation (CRYO) was founded in 2008, to allow individuals, researchers and physicians to collect-process-store stem cells derived from adipose tissue (fat) to prepare for their current or future use. Over the years the Company has become a biotechnology pioneer, standardizing adipose tissue derived technologies (Adult Stem Cells) for the fields of Regenerative and Personalized Medicine.

The Company operates a state-of-art, FDA-registered, clinical laboratory in New Jersey and licensed laboratories in Hong Kong, China and Tokyo, Japan, which operate on our proprietary platform, dedicated to the collection, processing, bio-banking, culturing and differentiation of adipose tissue (fat) and adipose derived stem cells (ADSCs)

CRYO maintains a strategic portfolio of intellectual property, 18 patents that surround the Companies proprietary technology which supports a growing pipeline of stem cell applications and biologic products. We are leveraging our proprietary platform and our developed product portfolio to create a domestic and global footprint of licensed laboratory affiliates, physicians networks and research organizations who purchase tissue collection, processing and storage services and consumables from the Company.

CRYO’s laboratory stem cell bank/line products are characterized adult human Mesenchymal Stem Cell (MSC’s) derived from adipose tissue that work in conjunction with our 13 patented (non-animal) medium lines.

The Company’s R&D efforts are focused on university and private collaborations to discover, develop and commercialize ADSC therapies by utilizing our standardized collection-processing-storage methodology and laboratory products combined with synergistic technologies to create jointly developed regenerative medicine applications and intellectual property.

MedicalResearch.com: What are stem cells?

Response: By definition, a stem cell is characterized by its ability to self-renew and its ability to differentiate along multiple lineage pathways. Ideally, a stem cell for regenerative medicinal applications should meet the following criteria:

1.) Can be found in abundant quantities (millions to billions of cells)

2.) Can be harvested by a minimally invasive procedure

3.) Can be differentiated along multiple cell lineage pathways in a regulateable and reproducible manner.

4.) Can be safely and effectively transplanted to either an autologous or allogeneic host

5.) Can be manufactured in accordance with current Good Manufacturing Practice guidelines

MedicalResearch.com: What are the advantages of gathering stem cells from fat tissue?  

Response: The main advantage of using adipose tissue (fat) derived stem cells compared with bone marrow-derived mesenchymal stem cells, is that adipose tissue-derived stromal cells (ADSC) do have an equal potential to differentiate into cells and tissues of mesodermal origin, such as adipocytes, cartilage, bone, and skeletal muscle. However, the easy and repeatable access to subcutaneous adipose tissue (fat) and the simple isolation procedures provide a clear advantage.

Bone marrow (BM) was the first source reported to contain MSCs. However, for clinical use, BM may be detrimental due to the highly invasive donation procedure and the decline in MSC number and differentiation potential with increasing age.

Adipose tissue (AT) is a source that can be obtained by a less invasive method and in larger quantities than bone marrow derived cells. It has been demonstrated that adipose tissue contains stem cells similar to bone marrow-MSCs, which are termed processed lipoaspirate cells. These cells can be isolated from cosmetic liposuctions in large numbers and grown easily under standard tissue culture conditions.

MedicalResearch.com: What are the main anticipated uses for fat-derived stem cells?

Response: A quick review of clinical trials.gov:

• Facial rejuvenation
• Hair Restoration Using Autologous Mesenchymal Stem Cells
• Facial Atrophy, Hair Disease
• Lipoatrophy;   Aging;   Wrinkles
• Abnormally Healing Wounds;   Scars;   Soft Tissue Defects
• Second- or Third-degree Burns
• Alopecia Areata;   Scarring Alopecia
• Rotator Cuff Tear – Partial Thickness
• Defect of Articular Cartilage;   Knee Osteoarthritis
• Tibial Fracture
• Knee Osteoarthritis
• Osteoarthritis;
• Neurodegenerative Diseases
• Chronic Obstructive Pulmonary Disease COPD
• Peripheral Arterial Disease
• Heart Failure, Coronary Disease
• Ischemic Heart Disease;   Coronary Arteriosclerosis;   Cardiovascular Disease;
• Non-Ischemic Congestive Heart Failure
• Acute Spinal Cord Injury
• Moderate to Severe Chronic Kidney Disease
• Degenerative Lesion of Articular Cartilage of Knee
• Diabetic Foot;   Venous Ulcer;   Pressure Ulcer
• Liver Cirrhosis
• Frailty Syndrome
• Stroke
• Type 1 and 2 Diabetes Mellitus
• Chronic Obstructive Pulmonary Disease
• Urinary Incontinence
• Low Back Pain
• Autism
• Brain Lesion (General)
• Dry Macular Degeneration
• Degenerative Disc Disease
• Parkinson’s Disease
• Multiple Sclerosis
• Severe Aplastic Anemia
• Spinal Cord Injury
• Rheumatoid Arthritis;
• Urinary Incontinence
• Alzheimer’s Disease
• Urticaria;   Autoimmune Diseases;   Immune System Diseases;   Skin Diseases
• Erectile Dysfunction
• Tennis Elbow

MedicalResearch.com: What have they been used for to date?

Response: Adipose derived stem cells are the subject of numerous clinical trials globally and are in used for regenerative medicine in locations where they are currently permitted by local government. Conditions that are currently treated or under investigative studies include joint injections for orthopedic purposes including osteoarthritis, sports injuries, degenerative joint disease (in lieu of joint replacement), pain and inflammatory treatments, in systemic disease studies are under way for treatment of Multiple Sclerosis, Muscular Dystrophy, Alzheimer’s, ALS, Parkinson’s disease, Crohn’s Disease, and other diseases with significant inflammatory components. Additionally, there have been and are continuing studies for organ and tissue repair caused by trauma or disease such as repair of heart tissue damage from heart attacks, kidney tissues for those suffering from kidney disease and lung and heart repair for those suffering from COPD and idiopathic pulmonary fibrosis. Currently there are many centers around the world administering autologous cells as a wellness treatment (anti-aging) for patients concerned with the general physical degeneration associated with aging.

MedicalResearch.com: Who might want to have stem cells retrieved and stored in this manner?

Response: Individuals that have family members suffering or who have died from degenerative or incurable diseases are the primary candidates for storing their cells for future use. Future use can include usage on a preventative basis (wellness) or to have younger and healthier cells available if unfortunately they become ill, have a traumatic injury, or are highly active and sustain a sports activity related injury. Additionally with the ongoing improvements in genetics and pharmacology individuals can have stored cellular samples tested with proposed medicines prior to use to ascertain effectiveness before exposing themselves to the side effects commonly found in pharmaceutical medicines.

MedicalResearch.com: What should readers take away from your report?

Response: Mainly that there is a growing body of evidence and scientific studies that there is an opportunity for individuals to use their own tissues and stem cells to heal and repair themselves by using their own cells. By storing and utilizing their own cells individuals can avoid the significant and potentially deadly reactions that can occur with donor cells and genetically modified cell therapies.

MedicalResearch.com: Is there anything else you would like to add? 

Response: Adult stem cells have a great potential to treat various diseases. For these cell-based therapies, adipose-derived stem cells (ADSCs) are one of the most promising stem cell types, and have taken center stage due to their pluripotency. ADSCs are not only easily obtained but also uniquely expandable. ADSCs can differentiate into adipocytes, osteoblasts, chondrocytes, myocytes and neurons under specific differentiation conditions, and these kinds of differentiation potential of ADSCs could be applied in regenerative medicine e.g., skin reconstruction, bone and cartilage formation, The emerging field of regenerative medicine and individuals will require a reliable source of stem cells in addition to biomaterial scaffolds and cytokine growth factors. Adipose tissue represents an abundant and accessible source of adult stem cells with the ability to differentiate along multiple lineage pathways. The isolation, characterization, and preclinical and clinical application of adipose-derived stem cells (ASCs)

The CRYO platform allows individuals to create multiple cellular applications from a single mini-lipo procedure by visiting one of approximately 200 plastic/cosmetic surgeons currently in the American CryoStem physician network.

As we age our stem cells age. There is no better time than today to store you stem cells.

• Peripheral Vascular Disease;   Ischemia;   Diabetic Foot
• Adipogenesis
• Lipodystrophy
• Complex Perianal Fistula
• Fecal Incontinence
• Ischemic Congestive Heart Failure
• Keratopathy
• Breast Reconstruction;   Contour Irregularities;   Volume Insufficiency
• Micromastia
• Breast Cancer
• Myocardial Infarction;   Coronary Arteriosclerosis;   Cardiovascular Disease;     Coronary Disease
• Systemic Lupus Erythematosus;
• Sharp’s Syndrome
• Delayed Graft Function
• Premature Ovarian Failure
• Recto-vaginal Fistula
• Extremely Complex Perianal Fistulae
• Infantile Spinal Muscular Atrophy, Type I
• Burn
• Coronary Artery Disease
• Bacterial Pneumonia
• Anal Fistula Wound, Nonpenetrating
• Dystrophy
• Ulcerative Colitis
• The Sequelae Caused by Severe Brain Injury
• Vocal Cord Paralysis, Unilateral
• Cerebellar Ataxia
• Hair Restoration Using Autologous Mesenchymal Stem Cells
• Dystrophic Epidermolysis Bullosa
• BPH;   Nocturia;   BPH With Urinary Obstruction;   Prostatism;   Prostate Inflammation
• Inflammatory Bowel Diseases
• Vocal Cord Dysfunction
• Hereditary Corneal Dystrophy;   Keratoconus
• Systemic Sclerosis
• Cardiac Disease
• Sepsis
• Osteoarthritis;   Erectile Dysfunction;   Autoimmune Diseases;   Cardiomyopathies;   Emphysema
• Degenerative Arthritis
• Lumbar Intervertebral Disc Degeneration
• Genetic High Risk for Schizophrenia
• Osteoarthritis;   Joint Disease;   Osteoarthritis,Knee;   Arthritis, Degenerative;   Osteoarthrosis;   Osteoarthrosis Deformans
• Soft Tissue Mass Removal

Disclosures: Please note that American Cryostem is publicly traded on the OTC. Ticker: CRYO

Note: Content is Not intended as an endorsement or as medical advice. Please consult your health care provider regarding your specific medical condition and questions.

Last Updated on August 25, 2017 by Marie Benz MD FAAD