Author Interviews, Infections / 09.03.2018
Wistar Scientists Delineate Critical Steps in Antibody Formation
MedicalResearch.com Interview with:
Chih-Chi Andrew Hu, Ph.D.
Associate professor in Microenvironment & Metastasis Program
Wistar Institute
MedicalResearch.com: What is the background for this study? What are the main findings?
Response: To help our body fight infections, B cells need to differentiate into plasma cells so that they can produce abundant antibodies against pathogens. Antibodies are folded and assembled in the endoplasmic reticulum (ER). Only those perfectly manufactured antibodies are allowed to be released from the ER and delivered to the outside of B cells to fight against the pathogens. IRE1 is a sensor protein that sits on the membrane of the ER, and can respond to B cell differentiation by activating the transcription factor called XBP1s. Activation of XBP1s allows B cells to expand the size of the ER and produce necessary chaperone proteins to help B cells manufacture perfect antibodies. By studying B cells that lack XBP1s, we discovered that these B cells produced dramatically increased levels of IRE1, and such IRE1 acquired phosphorylation at its serine 729 (S729). (more…)