Author Interviews, Cancer Research / 14.01.2016
Separate Roles For Protein Kinases in Embryo Development and Cancer
More on Protein Kinases on MedicalResearch.com
MedicalResearch.com Interview with:
Dr Angus Cameron
Kinase Biology Laboratory
Barts Cancer Institute at Queen Mary University of London and
Professor Peter Parker
Protein Phosphorylation Laboratory
The Francis Crick Institute
Medical Research: What is the background for this study? What are the main findings?
Response: A particular family of candidate targets for cancer therapies (the three members of the protein kinase, or PKN, family) have been largely overlooked in terms of our understanding of their roles in normal biology and also in disease.
Since any programme to develop new treatments is well informed by understanding the normal roles of the drug targets in non-tumour settings, our research teams ‘knocked-out’ these genes in a model mammalian system, in mice, and observed the effect.
A key finding is that whilst two of the protein kinases, PKN1 and PKN3, are not necessary for mammalian development or adulthood, for PKN2 there is an absolute requirement in embryo development, but evidence of little requirement in adulthood.
Our teams then analysed in some detail the genetics and pathology of the developmental defects for PKN2 knock-outs. This established the importance of PKN2 in mesenchymal cell survival and proliferation during development. Mesenchymal cells are dynamic contractile cells which provide structure and shape to the developing embryo. This finding has profound effects on multiple developmental events. Mesenchymal cells are also important in many connective tissue conditions, including fibrosis of the lungs, and in cancer where mesenchymal cells can support invasion of cancer cells into tissues.
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